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Strict Standards: Non-static method DB::isError() should not be called statically in /home/avtor123/avtor123.nichost.ru/docs/pages/showitem.php on line 8 KMK:Ïðàêòèêóì ïî ýíçèìîëîãèè. ×àñòü ïåðâàÿ. Ïðîòåîëèòè÷åñêèå ôåðìåíòû. , Àñååâ Â.Â., Ëåâèöêèé Ñ.À., Ðóäåíñêàÿ Þ.À., Ðóáöîâ Ì.À., Ðóäåíñêàÿ Ã.Í., Êàëåáèíà Ò.Ñ.
2019. ISBN 978-5-907213-60-9. 70 ñ., öâ. èë. Ôîðìàò 145 õ 210 ìì. Â îáë. Òèðàæ 250 ýêç. Öåíà 100 ðóá.
Ïðàêòèêóì ïîñâÿùåí îñâîåíèþ ìåòîäîâ ðàáîòû ñ ôåðìåíòàìè íà ïðèìåðå ïðîòåîëèòè÷åñêèõ ôåðìåíòîâ — ñåðèíîâûõ ïðîòåèíàç òèïà ñóáòèëèçèíà è òèïà òðèïñèíà: ðàçäåëåíèþ ñìåñè áåëêîâ, ñîäåðæàùåé ïðîòåèíàçó, ñ ïîìîùüþ âûñîêîýôôåêòèâíîé æèäêîñòíîé õðîìàòîãðàôèè (FPLC) íà èîíîîáìåííîì ñîðáåíòå è ñ ïîìîùüþ àôôèííîãî ñîðáåíòà, ïîñòðîåíèþ ïðîôèëåé ýëþöèè è îïðåäåëåíèþ óäåëüíîé è îáùåé àêòèâíîñòè ïðîòåèíàç ñ èñïîëüçîâàíèåì ñèíòåòè÷åñêèõ õðîìîãåííûõ è ïðèðîäíûõ ñóáñòðàòîâ, õàðàêòåðèñòèêå ýíçèìàòè÷åñêèõ ñâîéñòâ ýòèõ ôåðìåíòîâ.
 ðåçóëüòàòå çàíÿòèé íà ïðàêòèêóìå îáó÷àþùèåñÿ çíàêîìÿòñÿ ñ ðàñïðîñòðàíåíèåì ïðîòåèíàç â ïðèðîäå, èõ áèîëîãè÷åñêîé ðîëüþ, çíà÷åíèåì äëÿ ìåäèöèíû è ôóíäàìåíòàëüíûõ íàó÷íûõ èññëåäîâàíèé, à òàêæå ïîëó÷àþò ôóíäàìåíòàëüíûå ïðåäñòàâëåíèÿ î ïðèíöèïàõ ðàáîòû ñ áåëêàìè, îáëàäàþùèìè ôåðìåíòàòèâíîé àêòèâíîñòüþ, ïðèíöèïàõ è ìåòîäàõ âûäåëåíèÿ è î÷èñòêè ïðîòåèíàç.
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